张茹;郝文革;孙静;张文英;张力;

• 1:广州市妇女儿童医疗中心血液肿瘤科

摘要(Abstract)：

目的研究原癌基因Bmi-1在儿童急性淋巴细胞白血病(ALL)患者肿瘤细胞中表达。方法通过qRT-PCR测定64例儿童ALL患者和18例正常对照儿童骨髓细胞中Bmi-1基因的表达水平,并对19例儿童ALL治疗前后进行动态观察。结果64例患儿中,男:女比例为1.67:1(40:24);免疫分型以B细胞型为主,占75%(48/64);49例患儿BCR/ABL基因为阴性表达,占89%;ALL骨髓样品中均检测到Bmi-1表达,占100%(64/64),中位表达量1.070,正常对照组阳性样本中位表达量0.4788(t=305,P=0.002),其中56例(65.9%)Bmi-1表达较对照组上调2倍以上;治疗后完全缓解的患儿骨髓细胞的Bmi-1水平表达下降率达到73.7%(14/19)。提示Bmi-1可能作为一种预后指标(t=98.5,P=0.0446)。结论 Bmi-1在儿童ALL表达增高,治疗完全缓解后,Bmi-1水平下降,为进一步研究Bmi-1能否成为儿童ALL的预后指标打下基础。

关键词(KeyWords)： Bmi-1基因;急性淋巴细胞白血病,儿童;预后

Abstract:

Keywords:

基金项目(Foundation): 广东省省级科技计划项目(2013B021800030)

作者(Author): 张茹;郝文革;孙静;张文英;张力;

Email:

DOI:

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